Donor substrate specificity of bovine kidney gamma-glutamyltransferase

- Five aromatic or polycyclic aromatic glutathione conjugates were synthesized.
- Enzyme kinetic studies were performed for each conjugate as a γ-glutamyltransferase (GGT) donor substrate.
- All conjugates tested were substrates for GGT, with a possible trend towards higher Km values for bulkier substrates.
- These results clarify the ability of GGT to act in the biotransformation of aromatic compounds.