Monoclonal antibodies to mouse butyrylcholinesterase

► We present the first monoclonal antibodies against mouse BChE, cross-reacting with dog and one of them with rat BChE. ► Antibodies recognize different epitopes which could enable double sandwich ELISA. ► Antibodies capture mouse BChE from different tissues. ► Immunohistochemistry of tissue section can replace more problematic activity staining. ► We propose an efficient way to purify mouse BChE from small amount of biological sample.