کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5848840 | 1130674 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mitogen-activated protein kinase and Akt pathways are involved in 4-n-nonyphenol induced apoptosis in mouse Sertoli TM4 cells
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کلمات کلیدی
DCFH-DADMEM/F-12MDAp-p382′-7′-dichlorofluoresceinRT-PCRHRPCATDcfPI3KPBSc-Jun N-terminal kinase - C-Jun N-terminal kinaseEDCs - EDC هاERK1/2 - ERK1 / 2GSH-Px - GSH-PXMAPK - MAPKMTT - MTTp-Akt - P-AKTp-ERK1/2 - P-ERK1 / 2ROS - ROSAkt - آکتEndocrine-disrupting compounds - ترکیبات غشایی ناپایدارApoptosis - خزان یاختهایSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازphospho-Akt - فسفات آکتPhosphate buffered saline - فسفات بافر شورPhosphatidylinositol 3-kinase - فسفاتیدیلینواستیل 3-کینازPhospho-p38 - فسفو -38phospho-Erk1/2 - فسفو ارک 1/2Apes - مردابNonylphenol - نونیل فنولreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسHorseradish peroxidase - پراکسیداز هوررادیشmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenAlkylphenol polyethoxylates - پلی اتیل سدیم آلکیلفنولCatalase - کاتالازextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولیglutathione peroxidase - گلوتاتیون پراکسیدازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
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چکیده انگلیسی
Nonylphenol (NP) is considered an important environmental toxicant, which may disrupt male reproductive system. The aim of this study was to investigate 4-n-nonylphenol (4-n-NP) induced apoptosis and its related mechanism in mouse Sertoli cell line, TM4 cells. Our results showed that NP treatment (0.1, 1, 10, 20 and 30 μM) decreased cell viability and induced apoptosis in the cells, accompanied by alteration of Bcl-2 family mRNA expression, activation of caspases-3, release of Ca2+, and increase of reactive oxygen species (ROS) generation. Subsequently, it was found that the levels of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-PX) in the cells were markedly decreased, and maleic dialdehyde (MDA) content was increased by NP treatment. Then activation of the mitogen-activated protein kinases (MAPKs) pathways and inhibition of Akt pathway were simultaneously detected in NP challenged TM4 cells. Taken together, it was concluded that NP induced cytotoxicity and apoptosis in TM4 cells, and the apoptosis may be mediated via MAPKs and Akt pathways in addition to Ca2+ release and ROS generation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 39, Issue 2, March 2015, Pages 815-824
Journal: Environmental Toxicology and Pharmacology - Volume 39, Issue 2, March 2015, Pages 815-824
نویسندگان
Xiaozhen Liu, Shaoping Nie, Danfei Huang, Mingyong Xie,