کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5848878 | 1130674 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Naringin attenuates the cytotoxicity of hepatotoxin microcystin-LR by the curious mechanisms to OATP1B1- and OATP1B3-expressing cells
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کلمات کلیدی
3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromidePVDFpNPPMC-LROATPOATP1B1OATP1B3IC50FCSVmaxMTT - MTTp-nitrophenyl phosphate - p-نیترفنیل فسفاتROS - ROSstandard deviation - انحراف معیارminimum essential medium - حداقل حداقل مورد نیازmaximum velocity - حداکثر سرعتpolyvinylidene difluoride - دی فلوئورید پلی وینیلیدینfetal calf serum - سرم گوساله جنینDetoxification - سم زداییMEM - مامانMichaelis constant - مایکلز ثابت استmicrocystin-LR - میکروسیستین LRNaringin - نارانینگینhalf maximal inhibitory concentration - نیمه حداکثر غلظت مهاریProtein phosphatase - پروتئین فسفاتازOrganic anion transporting polypeptide - پلیپپتید حمل آنیون ارگانیکReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Microcystin-LR, which is an inhibitor of serine/threonine protein phosphatase (PP)1 and PP2A, induces liver injury by its selective uptake system into the hepatocyte. It is also thought that microcystin-LR induces reactive oxygen species (ROS). We tried to establish the chemical prevention of microcystin-LR poisoning. We investigated the effect of grapefruit flavanone glycoside naringin on cytotoxicity of microcystin-LR using human hepatocyte uptake transporter OATP1B3-expressing HEK293-OATP1B3 cells. We found cytotoxicity of microcystin-LR was attenuated by naringin in a dose dependent manner. The inhibition magnitude of total cellular serine/threonine protein phosphatase activity induced by microcystin-LR was suppressed by naringin. In addition, uptake of microcystin-LR into HEK293-OATP1B3 cells was inhibited by naringin. Furthermore, microcystin-LR induced phosphorylation of p53 was inhibited by naringin. Regardless of the difference in the exposure pattern of pre-processing and post-processing of naringin, the toxicity of microcystin-LR was comparable. These results suggested that naringin is promising remedy as well as preventive medicine for liver damage with microcystin-LR. In addition, involvement of ROS production after exposure to the sublethal concentrations of microcystin-LR in the onset of cytotoxicity was negligible. Therefore, inhibition of microcystin-LR uptake and the pathway other than ROS production would be involved in the effect of naringin on the attenuation of microcystin-LR toxicity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 39, Issue 2, March 2015, Pages 974-981
Journal: Environmental Toxicology and Pharmacology - Volume 39, Issue 2, March 2015, Pages 974-981
نویسندگان
Shota Takumi, Satoshi Ikema, Tamami Hanyu, Yusuke Shima, Takashi Kurimoto, Kazuhiro Shiozaki, Yasumasa Sugiyama, Ho-Dong Park, Seiichi Ando, Tatsuhiko Furukawa, Masaharu Komatsu,