کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5850251 | 1561775 | 2014 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Antrodia cinnamomea profoundly exalted the reversion of activated hepatic stellate cells by the alteration of cellular proteins
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کلمات کلیدی
PPAR-γp55Smc6Secretogranin IIaHSCsACMEMTSDAPIAntrodia cinnamomeaMMP-2PMSTAADMEMα-SMAFBS4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولROS - ROSalpha smooth muscle actin - آلفا آکتیو عضله صافReversion - بازگشتProteomic analysis - تجزیه پروتئومیکسThioacetamide - تیوات آمیدfetal bovine serum - سرم جنین گاوHepatic stellate cells - سلولهای ستارهای کبدیMass spectrometry - طیف سنجی جرمیactivated hepatic stellate cells - فعال سازی سلول ستون کبدیphenazine methosulfate - فنزین متوسولفاتmatrix metalloproteinase 2 - ماتریکس متالوپروتئیناز 2Dulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده Dulbeccostructural maintenance of chromosomes - نگهداری ساختاری کروموزوم هاliquid chromatography - کروماتوگرافی مایعPeroxisome proliferator-activated receptor gamma - گاما گیرنده گیرنده فعال پرولیفیزوم فعالReactive oxygen species - گونههای فعال اکسیژنtumor necrosis factor receptor 1 - گیرنده فاکتور نکروز تومور 1
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The direct modulation of Antrodia cinnamomea (AC) on the prominent role of liver fibrosis-hepatic stellate cells (HSCs) in situ remains unclear. Firstly, the administration of A. cinnamomea mycelial extract (ACME) could improve liver morphology and histological changes including collagen formation and GPT activity in the liver of thioacetamide (TAA)-injured rats. The morphology and fatty acid restore of TAA-induced HSCs (THSCs) returned to the non-chemical induced HSCs (NHSCs) type as measured by immunofluorescence and Oil Red O staining. PPARγ was upregulated associated with the lowering of α-SMA protein in NHSC-ACME. ACME inhibited the MMP-2 activity in NHSCs by gelatin Zymography. After LC-MS/MS, the cytoskeleton (tubulin, lamin A) and heat shock protein 8 in NHSC-ACME, and guanylate kinase, brain-specific kinase, SG-II and p55 proteins were downregulated in THSC-ACME. Whereas MHC class II, SMC6 protein, and phospholipase D were upregulated in NHSC-ACME. Furthermore, PKG-1 was downregulated in NHSC-ACME and upregulated in THSC-ACME. SG-II and p55 proteins were downregulated in NHSC-ACME and THSC-ACME by Western blotting. Taken together, the beneficial effect of A. cinnamomea on the induction of HSC cellular proteins is potentially applied as an alternative and complementary medicine for the prevention and amelioration of a liver injury.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 69, July 2014, Pages 150-162
Journal: Food and Chemical Toxicology - Volume 69, July 2014, Pages 150-162
نویسندگان
Yi-Ren Chen, Kai-Ting Chang, May-Jywan Tsai, Chia-Hung Lee, Kao-Jean Huang, Henrich Cheng, Yen-Peng Ho, Jian-Chyi Chen, Hsueh-Hui Yang, Ching-Feng Weng,