کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5856771 1131982 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of uremic serum and uremic toxins on drug metabolism in human microsomes
ترجمه فارسی عنوان
تأثیر سموم اورمیک و سمی اورمیک بر متابولیسم دارو در میکروسوم های انسانی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی


- No consistent effect on CYP3A4 or CYP2B6 by CRF patient serum pre-/post-dialysis.
- CMPF, hippuric acid and p-cresol had IC50 values achievable in CRF patient plasma
- IC50 values for indoxyl sulfate and indole-3-acetic acid above CRF plasma levels.

There is increasing evidence that renal impairment modifies nonrenal drug clearance through drug metabolizing cytochrome P450 (CYP) enzymes. In this study, the direct inhibitory effect of serum from chronic renal failure (CRF) patients receiving dialysis was evaluated in CYP3A4 (testosterone) and CYP2B6 (bupropion) metabolism assays. Human liver microsomes were incubated with ultrafiltered serum collected pre- and post-hemodialysis from ten CRF patients. Additionally, several uremic toxins were evaluated in the CYP3A4 assay. In only three patients was there a significant decrease or increase in testosterone or bupropion metabolism post-dialysis. Urea, mannitol, guanidine, homocysteine, uridine and creatinine had no effect on CYP3A4 metabolism. CMPF, hippuric acid and p-cresol had IC50 values that fell within CRF patient plasma concentrations. The IC50 values for indoxyl sulfate and indole-3-acetic acid were greater than CRF plasma concentrations. The lack of a consistent effect on CYP3A4 or CYP2B6 metabolism by uremic serum may be due in part to the frequency of hemodialysis in these patients which reduced the accumulation of uremic toxins. CMPF, hippuric acid and p-cresol have the ability to inhibit CYP3A4 metabolism at clinical concentrations which may correspond to reports of changes in hepatic metabolism in some CRF patients.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regulatory Toxicology and Pharmacology - Volume 68, Issue 2, March 2014, Pages 297-303
نویسندگان
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