کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5860552 1133190 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Beauvericin-induced cytotoxicity via ROS production and mitochondrial damage in Caco-2 cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Beauvericin-induced cytotoxicity via ROS production and mitochondrial damage in Caco-2 cells
چکیده انگلیسی


- BEA produced cytotoxic effects on Caco2 cells.
- Oxidative stress is involved in BEA toxicity.
- Apoptosis and mitochondrial disruption are produced after exposure to BEA on Caco 2 cells.
- DNA damage was observed after 12.0 μM BEA exposure.

The cytotoxicity of beauvericin (BEA) on human colon adenocarcinoma (Caco-2) cells was studied as a function of time. Moreover, the oxidative damage and cell death endpoints were monitored after 24, 48 and 72 h. After BEA exposure, the IC50 values ranged from 1.9 ± 0.7 to 20.6 ± 6.9 μM. A decrease in reduced glutathione (GSH; 31%) levels, as well as an increase in oxidized glutathione (GSSG, 20%) was observed. In the presence of BEA, reactive oxygen species (ROS) level was highly increased at an early stage with the highest production of 2.0-fold higher than the control that was observed at 120 min. BEA induced cell death by mitochondria-dependent apoptotic process with loss of the mitochondrial membrane potential (ΔΨm; 9% compared to the control), increase in LPO level (from 120% to 207% compared to the control) and reduced G0/G1 phase, with an arrest in G2/M, in a dose and time-dependent manner. Cell proliferation, apoptosis and ΔΨm determined, were in a dose- time-dependent manner. Moreover, DNA damage was observed after 12.0 μM concentration. This study demonstrated that oxidative stress is one of the mechanism involved in BEA toxicity, moreover apoptosis induction and loss of ΔΨm contribute to its cytotoxicity in Caco-2 cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 222, Issue 2, 24 October 2013, Pages 204-211
نویسندگان
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