کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5860596 | 1133201 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Vitamin C forestalls cigarette smoke induced NF-κB activation in alveolar epithelial cells
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کلمات کلیدی
NF-κBFACSPMNCSEDcfDCFH-DADAPI2′,7′-dichlorofluorescin - 2 '، 7'-dichlorofluorescin2′,7′-dichlorofluorescin diacetate - 2 '، 7'-dichlorofluorescin diacetate4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولROS - ROSElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزOxidative stress - تنش اکسیداتیوCigarette smoke - دود سیگارPolymorphonuclear cells - سلولهای پلیمورفونیکEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکcigarette smoke extract - عصاره سیگار سیگارnuclear factor kappa-B - فاکتور هسته ای کاپا-Bfluorescence activated cell sorter - فلورسانس سلول فعال شده سلولvitamin C - ویتامین سیReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Vitamin C forestalls cigarette smoke induced NF-κB activation in alveolar epithelial cells Vitamin C forestalls cigarette smoke induced NF-κB activation in alveolar epithelial cells](/preview/png/5860596.png)
چکیده انگلیسی
Cigarette smoking causes cellular oxidative stress resulting in inflammatory diseases of lung wherein transcription factor NF-κB plays an important role. It is possible that vitamin C, an antioxidant, may prevent cigarette smoke (CS)-induced NF-κB activation that involves degradation of I-κBε and nuclear translocation of c-Rel/p50 in alveolar epithelial cells. Therefore, to examine the hypothesis, we verified the effect of vitamin C on CS-induced expression of NF-κB driven luciferase reporter and NF-κB binding at its target DNA by EMSA in alveolar epithelial A549 cells. We also examined the level of I-κBε and sub-cellular distribution of c-Rel by western blotting and immunofluorescence respectively in CSE-treated A549 cells with or without vitamin C pretreatment. We observed a significant reduction in CSE induced luciferase expression, NF-κB DNA binding, I-κBε degradation and c-Rel nuclear translocation in cells pretreated with vitamin C. To further validate the result, we examined sub-cellular distribution of c-Rel in lungs of CS-exposed guinea pigs treated or untreated with vitamin C. Result showed that vitamin C treatment resulted in markedly reduced c-Rel nuclear translocation. All these results demonstrate that vitamin C prevents CS(E)-induced NF-κB activation and thus it could be used for the prevention of CS-induced inflammatory diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 220, Issue 1, 20 June 2013, Pages 76-81
Journal: Toxicology Letters - Volume 220, Issue 1, 20 June 2013, Pages 76-81
نویسندگان
Bannhi Das, Palash C. Maity, Alok K. Sil,