Transient knock down of checkpoint kinase 1 in hematopoietic progenitors is linked to bone marrow toxicity

► Bone marrow toxicity is one of the most common adverse effects observed in oncology drug development. ► Because of the critical roles kinases play during hematopoiesis, many kinase inhibitors are associated with bone marrow toxicity.► An effective method to knock down a gene of interest was established in hard-to-transfect hematopoietic stem cells. ► Our results support a direct role of Chk1 in maintaining normal hematopoiesis in the bone marrow.