کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5861218 1562713 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Herp depletion inhibits zearalenone-induced cell death in RAW 264.7 macrophages
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Herp depletion inhibits zearalenone-induced cell death in RAW 264.7 macrophages
چکیده انگلیسی
Herp is an endoplasmic reticulum (ER) membrane protein and strongly induced by the ER stress that not only participates in the unfolded protein response (UPR) under the ER stress, but also in cell autophagy under glucose starvation (GS). However, we do not know whether Herp plays any roles in other responses, such as zearalenone (ZEA). In this study, we constructed recombinant lentiviral vectors for Herp shRNA expression and generated stable Herp knockdown RAW 264.7 macrophages. Flow cytometry analysis showed Herp depletion could inhibit cell death induced by ZEA. Western blot analysis revealed that Herp depletion could up-regulate autophagy-related protein LC3-I conversion into LC3-II and the expression of ER stress-related protein CHOP. These results suggest that Herp depletion inhibits cell death by up-regulating autophagy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 32, April 2016, Pages 115-122
نویسندگان
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