کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5861878 | 1133767 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of SMP-028 on steroidogenesis in rats; mechanism of toxicological events on endocrine organs of rats
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DMEMFBSRIABSA - BSADMSO - DMSODulbecco’s modified Eagle medium - Modified Eagle اصلاح شده Dulbeccobovine serum albumin - آلبومین سرم گاوSteroidogenesis - استروئیدوژنزInhibition - بازداریDimethyl sulfoxide - دیمتیل سولفواکسیدradioimmunoassay - رادیوایمونواسیfetal bovine serum - سرم جنین گاوEndocrine toxicity - سمیت غدد درون ریزRat - موش صحرایی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effect of SMP-028 on steroidogenesis in rats; mechanism of toxicological events on endocrine organs of rats Effect of SMP-028 on steroidogenesis in rats; mechanism of toxicological events on endocrine organs of rats](/preview/png/5861878.png)
چکیده انگلیسی
SMP-028 is a new compound for treatment of asthma. Oral administration of SMP-028 to rats was associated with toxicological events in endocrine organs. These events mainly consisted of pathological changes in the adrenal gland, testis, prostate, seminal vesicle, ovaries, and uterus. In this study, we set to clarify whether SMP-028 inhibits steroidogenesis in primary culture cells obtained from rat endocrine organs in vitro. Adrenal cells, testicular cells, and ovarian cells were treated with SMP-028 and the production of steroid hormones, i.e., progesterone, aldosterone, corticosterone, total testosterone, and estradiol from these cells was measured by radioimmunoassay. We found that the production of progesterone from these cells treated with SMP-028 at 1 μM decreased to 16-67% that of the control. These findings indicate that SMP-028 inhibits steroidogenesis in rat endocrine organs in vitro. Considering that free maximum concentration in rats treated with SMP-028 are higher than the IC50 values for the inhibition of steroidogenesis in vitro, it is therefore believed that the toxicological events seen in rats following treatment with SMP-028 are due to inhibition of steroidogenesis in vivo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 28, Issue 3, April 2014, Pages 397-402
Journal: Toxicology in Vitro - Volume 28, Issue 3, April 2014, Pages 397-402
نویسندگان
Yohei Nishizato, Satoki Imai, Atsushi Yabunaka, Noriko Okahashi, Takeshi Kunimatsu, Masashi Yabuki,