Effects of palmitic acid on TNF-α-induced cytotoxicity in SK-Hep-1 cells

► An in vitro NASH model was created in SK-Hep-1 cells using palmitic acid and TNF-α. ► Palmitic acid promoted TNF-α-induced cytotoxicity by blocking survival signaling. ► This in vitro model may be used to screen drug candidates for the treatment of NASH.