A cell-based, multiparametric sensor approach characterises drug-induced cytotoxicity in human liver HepG2 cells

► An in vitro method allows to identify early onset of drug-induced cytotoxicity. ► Cellular oxygen consumption, acidification and impedance is monitored. ► Metabolic activity, cellular respiration and cell morphology is changed by drugs. ► A strategy is proposed to address potential risks for drug-induced cytotoxicity.