In vitro evaluation of cytochrome P450 induction and the inhibition potential of mitragynine, a stimulant alkaloid

► Mitragynine was evaluated for its effects on CYP1A2, CYP2D6 and CYP3A4 activities. ► It has no significant effects on CYP2D6. ► It induces mRNA and enzymatic activity of CYP1A2 significantly. ► It induces CYP3A4 weakly at the transcriptional level. ► It inhibits CYP3A4 weakly by interacting with the enzyme.