کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5862638 | 1133781 | 2011 | 6 صفحه PDF | دانلود رایگان |
Butenolide, a mycotoxin elaborated by several toxigenic Fusarium species, has been implicated as an etiological factor of Kashin-Beck disease and it is always detected in food from endemic Kashin-Beck disease areas. Although butenolide is considered as a potential health risk to humans and animals, its toxicity targets and mechanism of action have not been fully understood and the knowledge of its developmental toxicity is absent. The present study investigated butenolide embryotoxicity via an in vitro whole embryo culture system using rat embryos. Embryos exposed to butenolide at a concentration of 0.625Â mg/L showed and differentiation similar to that of the control embryos (=no observed adverse effect concentration; NOAECwec). The embryonic growth and differentiation were affected, represented as reduced crown-rump length and head length, and decreased number of somites from 1.25Â mg/L. Total morphological scores decreased significantly at the concentration of butenolide of 2.5Â mg/L. All embryos were malformed at 3.75Â mg/L and above (=ICMaxWEC), presenting growth retardation with flexion failure and irregular somite differentiation. The IC503T3 of butenolide as calculated from the balb/c 3T3 cytotoxicity test is 6.45Â mg/L. Our study shows that butenolide exerts detrimental effects on embryo development in vitro by inducing growth retardation and differentiation inhibition, and the embryotoxicity effect of butenolide should be treated with caution.
⺠The effects of butenolide on animal and human embryonic development are unknown. ⺠The embryotoxicity of butenolide is studied via an whole embryo culture model. ⺠Butenolide induces growth retardation and differentiation inhibition. ⺠The viability of 3T3-cell is impaired at 2.5 mg/L, and the IC50 3T3 is 6.45 mg/L. ⺠Butenolide exerts detrimental effects on rat embryo development in vitro.
Journal: Toxicology in Vitro - Volume 25, Issue 8, December 2011, Pages 1727-1732