Evaluation and validation of multiple cell lines and primary mouse macrophages to predict phospholipidosis potential

► PLD in cell lines and primary cells using LipidTox™ and NBDPE on HCS or platereader. ► PLD response similar irrespective of cell type, dye or platform. ► PLD rank order: primary mouse macrophages > RAW264.7 > I-13.35 > HepG2 ≈ HuH7. ► RAW264.7 cells selected as first-tier screen followed by primary macrophages.