Evaluation of topically applied copper(II) oxide nanoparticle cytotoxicity in human skin organ culture

► Production and characterization of 15-100 nm-diameter CuO nanoparticles. ► Use of organ culture to check topical toxicity of CuO nanoparticles in human skin. ► EM characterization of CuO nanoparticle cytotoxicity to human skin epidermis. ► Effects of CuO nanoparticles on vitality and inflammation biomarkers in skin tissue.