کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905123 | 1159836 | 2016 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparative transcriptome analyses indicate enhanced cellular protection against FMDV in PK15 cells pretreated with IFN-γ
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کلمات کلیدی
PARP-12′-5′-oligoadenylate synthetaseRLRsquantitative real-time reverse transcription PCRMDA5FMDVRIG-IFMDIFN-γDGEDEGsqRT-PCRFDRISGs - ISG هاMx1 - MX1TLRs - TLR هاdigital gene expression - بیان ژن دیجیتالFoot-and-mouth disease - تب برفکی، بیماری دهان و پاRNA sequencing - ترتیب RNAApoptosis - خزان یاختهایKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوfalse discovery rate - میزان کشف کاذبGene ontology - هستیشناسی ژنیOAS - واحهFoot and mouth disease virus - ویروس بیماری پا و دهانImmune response - پاسخ یا واکنش ایمنیpoly (ADP-ribose) polymerase-1 - پلی (ADP-ribose) پلیمراز-1melanoma differentiation-associated gene 5 - ژن 5 مرتبط با تمایز ملانوم 5retinoic acid-inducible gene I - ژن I القاء شده اسید رتینوئیکIFN-stimulated genes - ژن تحریک شده توسط IFNDifferentially expressed genes - ژن های متفاوت بیان شده استDifferently expressed genes - ژن های مختلف متفاوت هستندInterferon gamma - گاما اینترفرونToll-like receptors - گیرنده های پولی مانندRIG-I-like receptors - گیرنده هایی مانند RIG-I
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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![عکس صفحه اول مقاله: Comparative transcriptome analyses indicate enhanced cellular protection against FMDV in PK15 cells pretreated with IFN-γ Comparative transcriptome analyses indicate enhanced cellular protection against FMDV in PK15 cells pretreated with IFN-γ](/preview/png/5905123.png)
چکیده انگلیسی
Interferon gamma (IFN-γ) can induce a host antiviral response to foot and mouth disease virus (FMDV) in vivo and in vitro. To elucidate the mechanism of IFN-γ anti FMDV infection in host cells, high-throughput RNA sequencing was analyzed for systemic changes in gene expression profiles in PK15 cells infected by FMDV with or without IFN-γ pretreatment. More than 25 million reads, covering 1.2-1.5 Gb, were analyzed from each experiment panel. FMDV challenge altered the transcription of genes involved in positively and negatively regulating cell death or apoptosis; however, the expected immune suppression response was not obvious. IFN-γ pretreatment combined with FMDV infection normalized the increase in apoptosis. Furthermore, the transcription factors required for IFN-γ functioning, STAT1 and IRF1 were up-regulated by IFN-γ pretreatment and stimulated downstream IFN-stimulated genes (ISGs). These induced ISGs are mainly responsible for antigen processing, antigen presentation or antiviral defense. Interestingly, a synergistic effect on some ISGs, including OAS1, OAS2, MX1, MX2, RIG-I and IFIT1, was observed in the combined treatment compared to the IFN-γ treatment alone. The suggested effects identified by RNA sequencing were consistent with cellular morphology changes and confirmed by related protein markers. This is the first report exploring transcriptome alterations introduced by FMDV infection with or without IFN-γ pretreatment. The identified key host genes that control cell survival in vitro broaden our comprehensive understanding of how IFN-γ inhibits FMDV infection and may shed light on developing improved FMD control approaches.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 586, Issue 2, 25 July 2016, Pages 206-215
Journal: Gene - Volume 586, Issue 2, 25 July 2016, Pages 206-215
نویسندگان
Yin Fu, Zesen Zhu, Huiyun Chang, Zaixin Liu, Jing Liu, Huiyong Chen,