کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5907838 | 1160871 | 2013 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mapping the translation initiation landscape of an S. cerevisiae gene using fluorescent proteins
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Accurate and efficient gene expression requires that protein translation initiates from mRNA transcripts with high fidelity. At the same time, indiscriminate initiation of translation from multiple ATG start-sites per transcript has been demonstrated, raising fundamental questions regarding the rate and rationale governing alternative translation initiation. We devised a sensitive fluorescent reporter assay for monitoring alternative translation initiation. To demonstrate it, we map the translation initiation landscape of a Saccharomyces cerevisiae gene (RMD1) with a typical ATG sequence context profile. We found that up to 3%-5% of translation initiation events occur from alternative out-of-frame start codons downstream of the main ATG. Initiation from these codons follows the ribosome scanning model: initiation rates from different start sites are determined by ATG order, rather than their context strength. Genomic analysis of S. cerevisiae further supports the scanning model: ATG codons downstream rather than upstream of the main ATG tend to have higher context scores.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics - Volume 102, Issue 4, October 2013, Pages 419-429
Journal: Genomics - Volume 102, Issue 4, October 2013, Pages 419-429
نویسندگان
Tuval Ben-Yehezkel, Hadas Zur, Tzipy Marx, Ehud Shapiro, Tamir Tuller,