Physicochemical and morphological properties of size-controlled chitosan-tripolyphosphate nanoparticles

- Chitosan-tripolyphosphate nanoparticles were prepared through ionic-crosslinking.
- Control of particle size by selecting appropriate conditions.
- Influence of initial pH and salinity of chitosan solution on nanoparticles formation.
- Separation of aggregates from nanoparticles by centrifugation.
- Ultra-sonication reduced particle size but caused fragmentation on their structure.