Isobaric tagging-based quantification by mass spectrometry of differentially regulated proteins in synaptosomes of HIV/gp120 transgenic mice: Implications for HIV-associated neurodegeneration

► Synaptosomes from HIV/gp12 Tg mice tandem mass tag labeled for MS/MS analysis. ► Akt pathway identified as suppressed in HIV/gp12 Tg brains. ► Akt pathway also suppressed in human HIV associated neurocognitive disorders (HAND). ► Akt is S-nitrosylated in HIV/gp120 and HAND brain. ► MS/MS and pathway analysis is means to identify potential drug targets in disease.