Effects of alendronate on human osteoblast-like MG63 cells and matrix metalloproteinases

Alendronate at 10−5 M or higher was toxic to the cells. Alendronate at 10−8 to 10−6 M did not alter the expression of MMP-1, MMP-2, MT1-MMP or TIMP-2, as well as did not alter collagen degradation. Alendronate inhibited MMP-2 activity at 10−3 and 10−2 M in the zymograms. In conclusion, non-toxic levels of alendronate (10−8 to 10−6 M) did not alter MMP expression in MG63 cells or inhibit MMP-2 activity.