کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6052081 1583344 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Increased expression of Toll-like receptor-9 has close relation with tumour cell proliferation in oral squamous cell carcinoma
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
Increased expression of Toll-like receptor-9 has close relation with tumour cell proliferation in oral squamous cell carcinoma
چکیده انگلیسی

ObjectiveToll-like receptor-9 (TLR-9), a new member of the interleukin-1 receptor superfamily, was recently found to have a high level of expression in many carcinoma specimens. The objective of this study was to examine the TLR-9 expression and its role in tumour cell proliferation in oral squamous cell carcinoma.Materials and methodsWestern blot and immunohistochemistry were used to detect TLR-9 protein in oral squamous cell carcinoma (OSCC) Tca-8113 cell lines and clinical specimens (n = 60). The relationship between TLR-9 expression and clinicopathologic features was analysed. Cell proliferation and inflammatory chemokines secretion were tested by MTT and ELISA methods respectively.ResultsResults showed that TLR-9 expression level was higher in OSCC tissues than in paired adjacent normal tissues (P < 0.01), and the expression level of TLR-9 was significantly associated with tumour size (P = 0.001), tumour clinical stage (P = 0.003) and Ki-67 expression (P < 0.01). In vitro results also suggested that stimulation of Tca-8113 cells with TLR-9 agonist CpG-ODN could significantly increase tumour cell proliferation as well as subsequent IL-1α and IL-6 secretions (P < 0.01), which could be partially inhibited by usage of anti-TLR-9 protein.ConclusionsIt was therefore hypothesized that increased expression of TLR-9 may be of great value in assessing the development of OSCC, and could be used as a new target for OSCC prevention and therapy in future.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Oral Biology - Volume 56, Issue 9, September 2011, Pages 877-884
نویسندگان
, , , , , ,