Artificially synthesized helper/killer-hybrid epitope long peptide (H/K-HELP): Preparation and immunological analysis of vaccine efficacy

- Artificially synthesized OVA-H/K-HELP containing helper and killer epitopes exhibited an elevated vaccine efficacy.
- Peptide-linker appeared to be critical for preparing an efficient H/K-HELP vaccine.
- OVA-H/K-HELP inhibited tumor growth in both preventive and therapeutic tumor vaccine models.
- OVA-H/K-HELP induced effective and sustained antigen-presentation exerted by professional DC to activate Th1 cells and CTL.