Optimization of a Der p 2-based prophylactic DNA vaccine against house dust mite allergy

► In vivo delivery by electroporation of DNA encoding allergen drastically increases the production of specific antibodies but not the IFNg production. ► CpG motifs in DNA plasmid backbone do not improve the DNA vaccine efficiency. ► Homologous prime/boost vaccinations through electroporation using DNA encoding allergen prevent house dust mite allergy.