کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6117337 | 1591562 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Re-evaluation of the involvement of NK cells and C-type lectin-like NK receptors in modulation of immune responses by multivalent GlcNAc-terminated oligosaccharides
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کلمات کلیدی
SMCGZMBGranzyme BNKG2DSBAphorbol 12-myristate 13-acetateCLRN-acetyl-d-glucosamineGlcNAcPRFPMA - LDC هاSoybean agglutinin - آگلوتیین سویاAnti-tumor immunity - ایمنی ضد تومورNK cells - سلولهای NKNKT cells - سلولهای NKTMacrophages - ماکروفاژها،درشت خوارهاPAMAM dendrimer - پامام دندریمرPerforin - پرفورینpolyamidoamine dendrimer - پلی آمیدآمین دندریمر
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Recognition of glycosylation patterns is one of the basic features of innate immunity. Ability of C-type lectin-like receptors such as NKR-P1 to bind saccharide moieties has become recently a controversial issue. In the present study, binding assay with soluble fluorescently labeled recombinant rat NKR-P1A and mouse NKR-P1C proteins revealed apparently no affinity to the various neoglycoproteins. Lack of functional linkage between NKR-P1 and previously described saccharide binder was supported by the fact, that synthetic N-acetyl-d-glucosamine octabranched dendrimer on polyamidoamine scaffold (GN8P) did not change gene expression of NKR-P1 isoforms in C57BL/6 and BALB/c mice divergent in the NK gene complex (both in vitro and in vivo). Surprisingly, N-acetyl-d-glucosamine-coated tetrabranched polyamido-amine dendrimer specifically binds to NKT cells and macrophages but not to NK cells (consistently with changes in cytokine patterns). Despite the fact that GN8P has been tested as an immunomodulator in anti-cancer treatment animal models for many years, surprisingly no changes in cytokine profiles in serum relevant to anti-cancer responses using B16F10 and CT26 harboring mouse strains C57BL/6 and BALB/c are observed. Our results indicate possible indirect involvement of NK cells in GN8P mediated immune responses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunology Letters - Volume 156, Issues 1â2, NovemberâDecember 2013, Pages 110-117
Journal: Immunology Letters - Volume 156, Issues 1â2, NovemberâDecember 2013, Pages 110-117
نویسندگان
Valéria Grobárová, Veronika Benson, Daniel Rozbeský, Petr Novák, Jan Äerný,