کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6117346 | 1591562 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Low gene expression levels of activating receptors of natural killer cells (NKG2E and CD94) in patients with fulminant type 1 diabetes
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کلمات کلیدی
CTLA-4ICAFITCFulminant type 1 diabetesEMCGAPDHPBMCsGADnatural killer - (سلول های) کشنده طبیعیglutamic acid decarboxylase - glutamic acid dearboxylasecytotoxic T-lymphocyte antigen 4 - آنتی ژن T-lymphocyte سیتوتوکسی 4Human leukocyte antigen - آنتی ژن لوسکسی انسانHLA - آنتیژن گلبول سفید انسانیEncephalomyocarditis - انسفالومیوکاردیتMicroarray - ریزآرایهNK cell - سلول NKperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیphycoerythrin - فایکوئیریدینfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتmajor histocompatibility complex - مجموعه سازگاری بافتی اصلیMHC - مجموعه سازگاری بافتی اصلیglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Low gene expression levels of activating receptors of natural killer cells (NKG2E and CD94) in patients with fulminant type 1 diabetes Low gene expression levels of activating receptors of natural killer cells (NKG2E and CD94) in patients with fulminant type 1 diabetes](/preview/png/6117346.png)
چکیده انگلیسی
Fulminant type 1 diabetes is an independent subtype of type 1 diabetes characterized by extremely rapid onset and absence of islet-related autoantibodies. However, detailed pathophysiology of this subtype is poorly understood. In this study, a comprehensive approach was applied to understand the pathogenesis of fulminant type 1 diabetes. We determined the genes that were differentially expressed in fulminant type 1 diabetes compared with type 1A diabetes and healthy control, using gene expression microarray in peripheral blood cells. Using volcano plot analysis, we found reduced expression of killer cell lectin-like receptor subfamily C, member 3 (KLRC3) which encodes NKG2E, a natural killer (NK) cell activating receptor, in fulminant type 1 diabetes, compared with healthy controls. This difference was confirmed by real-time RT-PCR among NK-enriched cells. The expression of KLRD1 (CD94), which forms heterodimer with NKG2E (KLRC3), was also reduced in NK-enriched cells in fulminant type 1 diabetes. Furthermore, flow cytometry showed significantly lower proportion of NK cells among peripheral blood mononuclear cells (PBMCs) in fulminant type 1 diabetes than in healthy controls. In patients with fulminant type 1 diabetes, the relative proportion of NK cells correlated significantly with the time period between onset of fever to the appearance of hyperglycemic-related symptoms. We conclude the presence of reduced NK activating receptor gene expression and low proportion of NK cells in fulminant type 1 diabetes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunology Letters - Volume 156, Issues 1â2, NovemberâDecember 2013, Pages 149-155
Journal: Immunology Letters - Volume 156, Issues 1â2, NovemberâDecember 2013, Pages 149-155
نویسندگان
Shinsuke Nakata, Akihisa Imagawa, Yugo Miyata, Atsushi Yoshikawa, Junji Kozawa, Kohei Okita, Tohru Funahashi, Seiji Nakamura, Kenichi Matsubara, Hiromi Iwahashi, Iichiro Shimomura,