NSOM/QD-based fluorescence-topographic image fusion directly reveals nano-spatial peak-valley polarities of CD69 and CD71 activation molecules on cell-membrane fluctuations during T-cell activation

► NSOM/QD based fluorescence-topographic image fusion system. ► Direct visualization of the nanoscale distribution of cell surface molecules on cell membrane fluctuations. ► Most of CD69 were clustered as nano-domain and polarized in the peak of the cell-membrane fluctuations. ► Most of CD71 were clustered as nano-domain and polarized in the valley of the cell-membrane fluctuations.