Real-time PCR strategy for parasite quantification in blood and tissue samples of experimental Trypanosoma cruzi infection

► Real-time PCR is a tool for monitoring parasite load in experimental Trypanosoma cruzi infections. ► Curves of parasitemia were generated by real-time PCR and fresh blood examination in acute phase. ► Correlation observed between parasite load in blood and in heart tissue in acute and chronic phases. ► Highest CCL2, CCL5 and NO levels were coincident with higher load of blood and tissue parasites. ► Inflammatory mediators were reduced in chronic phase proportionally to the parasites control.