کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6132785 | 1593441 | 2016 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel recombinant Meq protein based dot-ELISA for rapid and confirmatory diagnosis of Marek's disease induced lymphoma in poultry
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کلمات کلیدی
IBHMEQRIPANCMALKPBSA - BSAbovine serum albumen - آلبومین سرم گاوAlkaline phosphatase - آلکالین فسفاتاز یا فسفاتاز قلیاییELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاDiagnosis - تشخیصHIS - خودRoom temperature - دمای اتاقNitrocellulose membrane - غشای نیتروسلولزLymphoma - لنفومLuria broth - لوریا بوثهPoultry - ماکیانDot-ELISA - نقطه الیزاInclusion body hepatitis - هپاتیت بدنیMarek’s disease virus - ویروس بیماری مارکPeroxidase - پراکسیداز
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Marek's disease (MD), is an economically important virus disease of poultry throughout the world. In this study, we for the first time reports development of a novel dot enzyme-linked immunosorbent assay (dot-ELISA) for the confirmatory diagnosis of lymphoma caused by Marek's Disease Virus (MDV). Suspected lymphoma tissue extracts from the diseased birds were used for the Meq oncoprotein antigen detection, which is expressed specifically in MDV lymphomas. Recombinant Meq oncoprotein was expressed using Expresso⢠Rhamnose Sumo Cloning and Expression system and the hyperimmune serum was raised against it, which was used later while developing dot-ELISA. The dot-ELISA exhibited higher specificity (92%) in diagnosing MD lymphomas as compared to conventional PCR (40%), where later assay is unable to differentiate disease development (lymphoma) and/or infection. The developed dot-ELISA proved to be a specific, rapid and inexpensive technique detecting MDV lymphomas in poultry. Of the note, this new assay could be opted as a valuable diagnostic tool in the resource poor countries andcould further be used to differentiate from other tumor causing viruses in poultry.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 236, October 2016, Pages 271-280
Journal: Journal of Virological Methods - Volume 236, October 2016, Pages 271-280
نویسندگان
M. Asok Kumar, R. Barathidasan, M. Palanivelu, S.D. Singh, Mohd Yaqoob Wani, Yashpal Singh Malik, R. Singh, K. Dhama,