کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6133055 | 1593452 | 2015 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection and discrimination of European isolates of Soil-borne wheat mosaic virus using immunocapture real-time reverse transcription-polymerase chain reaction
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کلمات کلیدی
RT-PCRRNA - اسید ریبونوکلئیکribonucleic acid - اسید ریبونوکلئیکELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاbase pair - جفت پایهReal-time RT-PCR - زمان واقعی RT-PCRreal-time reverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی معکوس و پلیمریزاکس معکوس در زمان واقعیreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسKilo base - پایه Kilocoat protein - پروتئین کت
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Soil-borne wheat mosaic virus (SBWMV) is a viral pathogen of cereal crops. Although first detected in the United States, it has since been reported in Germany and Poland. To date, two SBWMV strains (Nebraska-like and New-York-like types) have been detected. While the nucleotide sequences of the coat protein (CP) genes from the two strains show only 89% similarity, the deduced amino acid sequences of this region are identical. Therefore, the isolates cannot be differentiated serologically using the double antibody sandwich enzyme-linked immunosorbent assay. An immunocapture real-time reverse transcription-polymerase chain reaction (IC-real-time-RT-PCR) assay was developed for detection and discrimination of SBWMV strains based on the amplification of short CP fragments (â¼250 bp). The results showed distinct melting curve profiles related to each strain. The primer pairs gave similar melting points, with a Tm of 82.1 °C for Nebraska-like and 83.5 °C for New-York-like SBWMV strains. This work confirms that IC-real-time-RT-PCR is a valuable technique for rapid detection and discrimination of SBWMV strains.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 225, 1 December 2015, Pages 55-58
Journal: Journal of Virological Methods - Volume 225, 1 December 2015, Pages 55-58
نویسندگان
Katarzyna Trzmiel, Marzena Lewandowska,