کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6133864 | 1593480 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of the performance of four methods for detection of hepatitis B surface antigen and their application for testing 116,455 specimens
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
HBsAgCLSICMIAECLIAC50GICAelectrochemiluminescent immunoassay - آزمایش ایمنی الکتروکوهیمیلومینسنتhepatitis B virus surface antigen - آنتی ژن سطح ویروسی هپاتیت BPerformance evaluation - ارزیابی یا سنجش عملکردChemiluminescent microparticle immunoassay - ایمونوکلئیک میکرومولکائین شیمیاییELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاMethod - روشClinical and Laboratory Standards Institute - موسسه استاندارد های بالینی و آزمایشگاهیHBV - هپاتیت بhepatitis B virus - ویروس هپاتیت بی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Hepatitis B surface antigen (HBsAg) is a crucial serum marker for the diagnosis of hepatitis B virus (HBV) infection. It is imperative to compare test results from different detection methods based on different principles. Four methods, chemiluminescent microparticle immunoassay (CMIA), electrochemiluminescent immunoassay (ECLIA), enzyme-linked immunosorbent assay (ELISA) and golden immunochromato-graphic assay (GICA) were applied to test the HBsAg level in 250 specimens. According to the EP12-A2 and EP15-A2 documents from Clinical and Laboratory Standards Institute (CLSI), the concentration at which repeated results are 50% positive (C50) of HBsAg detected by CMIA, ECLIA, ELISA and GICA was 0.05, 0.08, 0.15 and 15.0 IU/ml, respectively. When the detection concentration of HBsAg was 0.5 IU/ml, the imprecision degree of CMIA, ECLIA and ELISA was 8.1%, 5.9% and 14.9% respectively. When detecting high HBsAg level (â¥20.0 IU/ml) and HBsAg negative specimens, the consistency of the four methods was high, while for the low level (0.05-20.0 IU/ml), the consistency was poor (except for the CMIA and ECLIA, P < 0.05). When evaluation of the four methods in qualitative diagnosis of HBsAg level in the 116,455 specimens, there was no significant discrepancy among CMIA, CMIA and ECLIA, however, GICA was significantly different from the other 3 methods. Compared with CMIA, the false negative rate of ECLIA, ELISA and GICA was 0.2%, 1.3% and 12.3% respectively. In conclusion, GICA was only suitable for the preliminary screening of HBsAg positive individuals and ELISA can be applied to the qualitative diagnosis of HBsAg. Both CMIA and ECLIA were suitable for the quantitative determination of HBsAg.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 196, February 2014, Pages 174-178
Journal: Journal of Virological Methods - Volume 196, February 2014, Pages 174-178
نویسندگان
Can Liu, Tianbin Chen, Jinpiao Lin, Huijuan Chen, Jing Chen, Sheng Lin, Bin Yang, Hongyan Shang, Qishui Ou,