کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6134225 | 1593482 | 2013 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of commercialized rapid diagnostic testing for some Hepatitis B biomarkers in an area of intermediate endemicity
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کلمات کلیدی
Rapid diagnostic testingRDTHBsAgPPVDNA - DNA یا اسید دزوکسی ریبونوکلئیکHepatitis B surface antigen - آنتی ژن سطحی هپاتیت Benzyme linked immunosorbent assay - آنزیم تست ایمونوسیورسانس مرتبط استpositive predictive value - ارزش پیش بینی مثبتnegative predictive value - ارزش پیش بینی منفیdeoxyribonucleic acid - اسید deoxyribonucleicELISA - تست الیزاhepatitis B surface antibody - سطح آنتی بادی هپاتیت Banti-HBc - ضد HBcAnti-HBe - ضد HBeAnti-HBs - ضد HBsReliability - قابلیت اطمینانNPV یا negative predictive value - مقادیر پیش بینی شده منفیHBV - هپاتیت بhepatitis B virus - ویروس هپاتیت بی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Hepatitis B virus (HBV) is a major public health threat. Enzyme immune assay (EIA) of HBsAg is the screening method used in most settings, including in blood banks. Other markers are used to evaluate the HBV replication, immunity and the infectious level of the patient. Testing negative for HBsAg, however, does not always mean the absence of infection, and testing other markers using EIA is costly. This study evaluated the diagnostic reliability of commercially available non-HBsAg HBV biomarkers to detect their usefulness to screen for HBV infection. INTEC rapid tests for HBV markers were evaluated in 508 HBsAg negative blood donors and were compared to EIA as a reference method. Only anti-HBs, anti-HBc and anti-HBe could be evaluated. Sensitivities of all tests (64.2, 85.48, and 82.78 respectively) were much lower than those claimed by the manufacturer. The specificities and negative predictive values for all tests exceeded 95% and 93% respectively and were lowest for anti-HBs. Anti-HBe had the highest accuracy (99.02%), while anti-HBs had the lowest (90.16%). It was concluded that the rapid test performance was lower than the manufacturer's reports. They are reliable tools to prove negativity, but less efficient to confirm reactivity. The best performance was for anti-HBe. The positive results of anti-HBs was significantly associated with higher ELISA titer levels, which is therefore recommended to be used for screening of immunity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 194, Issues 1â2, December 2013, Pages 190-193
Journal: Journal of Virological Methods - Volume 194, Issues 1â2, December 2013, Pages 190-193
نویسندگان
Engy Mohamed El-Ghitany, Azza Galal Farghaly,