Quantitative and sensitive detection of the SARS-CoV spike protein using bispecific monoclonal antibody-based enzyme-linked immunoassay

► Two monoclonal antibodies against SARS CoV S1 antigen were generated by hybridomatechnology. ► Bispecific antibody (bsmAb) was developed by Quadroma technology. ► Mono- and bispecific antibodies were evaluated by sandiwichELISA for immunodiagnosis. ► We report that bsmAb-based ELISA is more sensitive than the mAb-based ELISA for detection of the S1 antigen.