Evaluation of envelope glycoprotein Erns of an atypical bovine pestivirus as antigen in a microsphere immunoassay for the detection of antibodies against bovine viral diarrhea virus 1 and atypical bovine pestivirus

► Atypical pestiviral Erns protein was produced in a baculovirus expression system. ► An Erns microsphere immunoassay was evaluated for detecting BVDV antibodies in 596 sera. ► The assay had a 100% sensitivity and specificity relative to ELISA.