کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6135987 | 1224920 | 2012 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Japanese encephalitis virus structural and nonstructural proteins expressed in Escherichia coli induce protective immunity in mice Japanese encephalitis virus structural and nonstructural proteins expressed in Escherichia coli induce protective immunity in mice](/preview/png/6135987.png)
Ectodomain of Japanese encephalitis virus (JEV) E protein [domains I through III (D1-3), domains I and II (D1-2) and domain III (D3)] and the nonstructural protein 1 (NS1) were expressed in Escherichia coli, and administered to BALB/c mice via the intranasal (i.n.) route. The E protein, but not the NS1, induced JEV-specific serum IgG with virus-neutralization capacity in vitro. When mice were lethally challenged with JEV, i.n. immunization with D1-3, D1-2, D3, or a mouse brain-derived formalin-inactivated JE vaccine conferred complete protection, while an 80% protection rate was observed in the NS1 immunized mice. Cytokine analysis of the cervical lymph nodes of mice i.n. immunized with D1-3 or NS1 revealed antigen-specific IL-2 and IL-17 responses, but no IFN-γ T cell response, were observed. This study demonstrates for the first time the i.n. vaccine efficacy of the E. coli-expressed recombinant JEV proteins.
Journal: Microbes and Infection - Volume 14, Issue 2, February 2012, Pages 169-176