کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6136122 | 1224973 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interaction of epithelial cell membrane rafts with Paracoccidioides brasiliensis leads to fungal adhesion and Src-family kinase activation
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کلمات کلیدی
PBSpolyvinylidene-difluoride4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-oneTNEERK MAP kinases3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromidePD98059Paracoccidioides brasiliensisPP2DRMSFKTBSTMβCDDMEMDAPICTBHRPPVDF2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride - 2- (4-آمیدوفنیل) -6-اندولکربامیدین دی هیدروکلریدBSA - BSADMSO - DMSODulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoERK1/2 - ERK1 / 2Src-family kinases - kinases خانواده SrcMTT - MTTp-ERK1/2 - P-ERK1 / 2bovine serum albumin - آلبومین سرم گاوHost-pathogen interactions - تعامل میزبان-پاتوژنdimethyl-sulfoxide - دی متیل سولفوکسیدdetergent-resistant membrane - غشای مقاوم در برابر مواد شویندهPhosphate buffered saline - فسفات بافر شورphospho-Erk1/2 - فسفو ارک 1/2methyl-β-cyclodextrin - متیل-β-سیکلوکودکسترینMembrane microdomains - میکرو دامین های غشاییHorseradish peroxidase - پراکسیداز هوررادیشAdhesions - چسبندگیcholera toxin subunit B - کلسترول توکسین Bextracellular signal-regulated kinase 1/2 - کیناز 1/2 تنظیم سیگنال خارج سلولیSrc-family kinase - کیناز Src-family
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Interaction of epithelial cell membrane rafts with Paracoccidioides brasiliensis leads to fungal adhesion and Src-family kinase activation Interaction of epithelial cell membrane rafts with Paracoccidioides brasiliensis leads to fungal adhesion and Src-family kinase activation](/preview/png/6136122.png)
چکیده انگلیسی
Membrane rafts are cholesterol- and sphingolipid-enriched cell membrane domains, which are ubiquitous in mammals and play an essential role in different cellular functions, including host cell-pathogen interaction. In this work, by using several approaches, we demonstrated the involvement of epithelial cell membrane rafts in adhesion process of the pathogenic fungus Paracoccidioides brasiliensis. This conclusion was supported by the localization of ganglioside GM1, a membrane raft marker, at P. brasiliensis-epithelial cell contact sites, and by the inhibition of this fungus adhesion to host cells pre-treated with cholesterol-extractor (methyl-β-cyclodextrin, MβCD) or -binding (nystatin) agents. In addition, at a very early stage of P. brasiliensis-A549 cell interaction, this fungus promoted activation of Src-family kinases (SFKs) and extracellular signal-regulated kinase 1/2 (ERK1/2) of these epithelial cells. Whereas SFKs were partially responsible for activation of ERK1/2, membrane raft disruption with MβCD in A549 cells led to total inhibition of SFK activation. Taking together, these data indicate for the first time that epithelial cell membrane rafts are essential for P. brasiliensis adhesion and activation of cell signaling molecules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbes and Infection - Volume 10, Issue 5, April 2008, Pages 540-547
Journal: Microbes and Infection - Volume 10, Issue 5, April 2008, Pages 540-547
نویسندگان
Paloma K. Maza, Anita H. Straus, Marcos S. Toledo, Helio K. Takahashi, Erika Suzuki,