کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6139312 | 1594234 | 2015 | 11 صفحه PDF | دانلود رایگان |
- When co-expressed with helper virus in N. benthamiana but not in N. tabacum, STMV generates a truncated RNA.
- A highly conserved 3â² stem-loop structure is obligatory for STMV translation.
- STMV is competent to form empty VLPs without helper virus.
- STMV assembly requires a 3â² tRNA-like structure.
In this study, we assembled an Agrobacterium-based transient expression system for the ectopic expression of Satellite tobacco mosaic virus (STMV) (+) or (â) transcripts and their biological activity was confirmed when Nicotiana benthamiana plants were co-expressed with helper Tobacco mosaic virus replicase. Characterization of STMV in the presence and absence of its HV revealed: (i) HV-dependent expression of STMV (+) in N. benthamiana, but not in N. tabacum, generated a replication-deficient but translation and encapsidation competent variant lacking the highly conserved 3â² 150 nucleotides (nt) (STMVÎ150); (ii) mutational analysis demonstrated that a conserved 3â² stem-loop structure in wild type and STMVÎ150 located between nt 874 and 897 is essential for translation of CP; (iii) helper virus-independent expression of CP from wt STMV was competent for the assembly of empty aberrant virion-like particles; whereas, CP translated from STMVÎ150 resulted in disorganized CP aggregates suggesting a role for the 3â²tRNA-like structure in STMV assembly.
Journal: Virology - Volume 483, September 2015, Pages 163-173