Knockdown of MAP4 and DNAL1 produces a post-fusion and pre-nuclear translocation impairment in HIV-1 replication

► DNAL1 and MAP4 play a role in post-fusion events of HIV-1 infection. ► DNAL1 and MAP4 knockdown impairs HIV-1 reverse transcription. ► DNAL1 and MAP4 do not mediate nuclear translocation. ► DNAL1 and MAP4 knockdown does not disrupt cytoskeletal organization.