Engagement of new castle disease virus (NDV) matrix (M) protein with charged multivesicular body protein (CHMP) 4 facilitates viral replication

► NDV M protein interacts with charged multivesicular body protein (CHMP) 4B and 4C in the cytoplasm. ► Knockdown of CHMP4B markedly inhibits NDV replication. ► Transient expression of CHMP4B/C dominant negative forms inhibits NDV replication. ► Cellular CHMP4s play a critical role in NDV replication.