کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6155827 | 1597940 | 2016 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Precision-cut human kidney slices as a model to elucidate the process of renal fibrosis
ترجمه فارسی عنوان
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کلمات کلیدی
BcrpHSP47FN1COL1A1ECMpdgfbGAPDHAdenosine Triphosphate - آدنوزین تری فسفاتATP - آدنوزین تری فسفات یا ATPperiodic acid–Schiff - اسید فسفریک SchiffPAI - باباplasminogen activator inhibitor - بازدارنده فعال کننده پلاسمینوژنchronic kidney disease - بیماری مزمن کلیویESRD یا end stage renal disease - بیماری کلیوی در مرحله نهایی Oat - جو دو سرorganic anion transporter - حمل کننده آنیون آلیfibronectin 1 - فیبرنکتین 1lactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Extracellular matrix - ماتریکس خارج سلولیCKD - نارسایی مزمن کلیهPAS - نهHeat shock protein 47 - پروتئین شوک حرارت 47breast cancer resistance protein - پروتئین مقاومت به سرطان سینهhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کاراglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
پزشکی و دندانپزشکی (عمومی)
چکیده انگلیسی
Chronic kidney disease is a major health concern, and experimental models bridging the gap between animal studies and clinical research are currently lacking. Here, we evaluated precision-cut kidney slices (PCKSs) as a potential model for renal disease. PCKSs were prepared from human cortical tissue obtained from tumor nephrectomies and cultured up to 96 hours. Morphology, cell viability, and metabolic functionality (ie, uridine 5'-diphospho-glucuronosyltransferase and transporter activity) were determined to assess the integrity of PCKSs. Furthermore, inflammatory and fibrosis-related gene expressions were characterized. Finally, to validate the model, renal fibrogenesis was induced using transforming growth factor β1 (TGF-β1). Preparation of PCKSs induced an inflammatory tissue response, whereas long-term incubation (96 hours) induced fibrogenesis as shown by an increased expression of collagen type 1A1 (COL1A1) and fibronectin 1 (FN1). Importantly, PCKSs remained functional for more than 48 hours as evidenced by active glucuronidation and phenolsulfonphthalein uptake. In addition, cellular diversity appeared to be maintained, yet we observed a clear loss of nephrin messenger RNA levels suggesting that our model might not be suitable to study the role of podocytes in renal pathology. Moreover, TGF-β1 exposure augmented fibrosis, as illustrated by an increased expression of multiple fibrosis markers including COL1A1, FN1, and α-smooth muscle actin. In conclusion, PCKSs maintain their renal phenotype during culture and appear to be a promising model to investigate renal diseases, for example, renal fibrosis. Moreover, the human origin of PCKSs makes this model very suitable for translational research.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Translational Research - Volume 170, April 2016, Pages 8-16.e1
Journal: Translational Research - Volume 170, April 2016, Pages 8-16.e1
نویسندگان
Elisabeth G.D. Stribos, Theerut Luangmonkong, Anna M. Leliveld, Igle J. de Jong, Willem J. van Son, Jan-Luuk Hillebrands, Marc A. Seelen, Harry van Goor, Peter Olinga, Henricus A.M. Mutsaers,