کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6271570 | 1614766 | 2015 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adverse effects of a SOD1-peptide immunotherapy on SOD1G93A mouse slow model of amyotrophic lateral sclerosis
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کلمات کلیدی
PBSIgGionized calcium binding adaptor moleculeSOD1OFTGFAPmotoneuronPFAα-SynKLHIba-1 - IBA-1α-synuclein - α-سینوکلینopen-field test - آزمایشی بازamyotrophic lateral sclerosis - اسکلروز جانبی آمیوتروفیکfamilial amyotrophic lateral sclerosis - اسکلروز جانبی جانبی آمیوتروفی فامیلیNeuroinflammation - التهاب عصبیimmunotherapy - ایمونوتراپیimmunoglobulin G - ایمونوگلوبولین Gphosphate buffer - بافر فسفاتALS - بیماری اسکلروز جانبی آمیوتروفیکELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاfALS - جعل اسنادsuperoxide dismutase 1 - سوپر اکسید دیسموتاز 1Superoxide dismutase - سوکسوکس دیسموتازPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMicroglia - میکروگلیاهاClinical score - نمره بالینیwild type - نوع وحشیparaformaldehyde - پارافرمالدهیدGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالkeyhole limpet hemocyanin - کلم هول limpet هموسیانین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Previous reports from our lab had shown that some anti-purinergic receptor P2X4 antibodies cross-reacted with misfolded forms of mutant Cu/Zn superoxide dismutase 1 (SOD1), linked to amyotrophic lateral sclerosis (ALS). Cross-reactivity could be caused by the abnormal exposure of an epitope located in the inner hydrophobic region of SOD1 that shared structural homology with the P2X4-immunizing peptide. We had previously raised antibodies against human SOD1 epitope mimicked by the P2X4 immunizing peptide. One of these antibodies, called AJ10, was able to recognize mutant/misfolded forms of ALS-linked mutant SOD1. Here, we used the AJ10 antigen as a vaccine to target neurotoxic species of mutant SOD1 in a slow mouse model of ALS. However, the obtained results showed no improvement in life span, disease onset or weight loss in treated animals; we observed an increased microglial neuroinflammatory response and high amounts of misfolded SOD1 accumulated within spinal cord neurons after AJ10 immunization. An increase of immunoglobulin G deposits was also found due to the treatment. Finally, a significantly worse clinical evolution was displayed by an impairment on motor function as a consequence of AJ10 peptide immunization.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 310, 3 December 2015, Pages 38-50
Journal: Neuroscience - Volume 310, 3 December 2015, Pages 38-50
نویسندگان
J. Sábado, A. Casanovas, H. Rodrigo, G. Arqué, J.E. Esquerda,