کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6271866 | 1614773 | 2015 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Tanshinone IIA protects dopaminergic neurons against 6-hydroxydopamine-induced neurotoxicity through miR-153/NF-E2-related factor 2/antioxidant response element signaling pathway
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
6-OHDAGCLMGCLCNQO-1Heme oxygenase-1Cyt CDOPACDAPIDCFH-DAHUVECS6-HydroxydopamineHO-1GSHNrf22′,7′-dichlorodihydrofluorescein diacetate - 2 '، 7'-dichlorodihydrofluorescein diacetate4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولBSA - BSADMSO - DMSOHPLC-MS/MS - HPLC-MS / MSMTT - MTTROS - ROSsiRNA - siRNAbovine serum albumin - آلبومین سرم گاوhigh-performance liquid chromatography-tandem mass spectrometry - اسپکترومتری جرمی کروماتوگرافی مایع با کارایی بالاParkinson’s disease - بیماری پارکینسونsmall interference RNA - تداخل کوچک RNADopamine - دوپامینdihydroxyphenylacetic acid - دی هیدروکسی فنیل اسیدهای اسیدDimethylsulfoxide - دیمتیل سولفواکسیدHuman umbilical vein endothelial cells - سلول های اندوتلیالی ورید ناف انسانcytochrome c - سیتوکروم سیNF-E2-related factor 2 - عامل NF-E2 2antioxidant response element - عنصر پاسخ آنتی اکسیدانlactic dehydrogenase - لاکتات دهیدروژنازLDH - لاکتات دهیدروژناز به صورت مختصر شده LDH ARE - هستندhomovanillic acid - هومووانیلیک اسیدHVA - چهglutamate cysteine ligase modifier subunit - کلرید کلسیم،Glutamate cysteine ligase catalytic subunit - کلوتامین کاتالیزوری لیستاز سیستئین گلوتاماتGlutathione - گلوتاتیونReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Parkinson's disease (PD) is the second most common progressive neurodegenerative disorder with increased oxidative stress, the underlying vital process contributing to cell death. Tanshinone IIA (Tan IIA), a major bioactive diterpene quinone of Salva miltiorrhiza, had been proved effective in the MPTP model through its anti-inflammatory activity. Here in this research, we found that Tan IIA prevented the loss of nigrostriatal dopaminergic neurons by activating the NF-E2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway. The cytotoxicity of 6-hydroxydopamine (6-OHDA) was attenuated by the treatment of Tan IIA in SH-SY5Y cells, which significantly reduced 6-OHDA-induced lactic dehydrogenase release and reactive oxygen species production. Further study indicated that Tan IIA contributed to the nuclear accumulation of Nrf2, which bound to the ARE sequence, and activated ARE-regulated genes, including heme oxygenase-1, glutamate cysteine ligase catalytic subunit (GCLC) and glutamate cysteine ligase modifier subunit (GCLM). Tan IIA also protected against damage to mitochondrial membrane potential, reduced the translocation of cytochrome c from the mitochondria to the cytoplasm and the activation of Caspase-9 and Caspase-3. Moreover, we demonstrated the above effects were performed in Nrf2-dependent manner. Further studies revealed that Tan IIA reduced the enhancement of miR-153 by 6-OHDA, which targeted the 3â²-UTR of Nrf2, and suppressed its expression and activation. Additionally, neurodegeneration caused by in vivo stereotaxic injection of 6-OHDA could also be ameliorated by the administration of Tan IIA. Taken together, our results strongly suggest that Tan IIA may be beneficial for the treatment of PD, and also confirm that targeting the Nrf2/ARE pathway is a promising strategy for therapeutic intervention in PD.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 303, 10 September 2015, Pages 489-502
Journal: Neuroscience - Volume 303, 10 September 2015, Pages 489-502
نویسندگان
X.S. Zhang, S. Ha, X.L. Wang, Y.L. Shi, S.S. Duan, Z.A. Li,