کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6276873 | 1295745 | 2010 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mesenchymal stem cells inhibition of chronic ethanol-induced oxidative damage via upregulation of phosphatidylinositol-3-kinase/Akt and modulation of extracellular signal-regulated kinase 1/2 activation in PC12 cells and neurons
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کلمات کلیدی
ERKMSC-CMMDATBSTNGFGSHbFGFMSCsDMEMFBSPI3K3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideBDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز DMSO - DMSOMAPKs - MAPK هاDulbecco’s modified Eagle medium - Modified Eagle اصلاح شده DulbeccoMTT - MTTp-Akt - P-AKTp-ERK1/2 - P-ERK1 / 2ROS - ROSChronic ethanol - اتانول مزمنAntioxidant action - اقدام آنتیاکسیدانیstandard deviation - انحراف معیارminimal essential medium - حداقل وسایل ضروریDimethyl sulfoxide - دیمتیل سولفواکسیدSOD - سدfetal bovine serum - سرم جنین گاوPC12 cells - سلول های PC12Mesenchymal stem cells - سلول های بنیادی مزانشیمیSuperoxide dismutase - سوکسوکس دیسموتازnerve growth factor - فاکتور رشد عصبbasic fibroblast growth factor - فاکتور رشد فیبروبلاست پایهBrain-derived neurotrophic factor - فاکتور نوروتروفی مشتق شده از مغزphospho-Akt - فسفات آکتphosphatidylinositol-3-kinase - فسفاتیدیلینواستیل-3-کینازphospho-Erk1/2 - فسفو ارک 1/2lactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH malondialdehyde - مالون دی آلدهیدMEM - مامانSignal transduction pathways - مسیرهای انتقال سیگنالPrimary cortical neurons - نورونهای قشر اولیهPropidium iodide - پروتئین یدیدreduced glutathione - کاهش گلوتاتیونextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولیmitogen-activated protein kinases - کیناز پروتئین فعال MitogenReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Mesenchymal stem cells inhibition of chronic ethanol-induced oxidative damage via upregulation of phosphatidylinositol-3-kinase/Akt and modulation of extracellular signal-regulated kinase 1/2 activation in PC12 cells and neurons Mesenchymal stem cells inhibition of chronic ethanol-induced oxidative damage via upregulation of phosphatidylinositol-3-kinase/Akt and modulation of extracellular signal-regulated kinase 1/2 activation in PC12 cells and neurons](/preview/png/6276873.png)
چکیده انگلیسی
It is well known that chronic ethanol consumption damages CNS through oxidative stress which results in many dysfunctions. Recently, it has been demonstrated that as a promising strategy to treat several neurological diseases, transplanted bone marrow-derived mesenchymal stem cells (MSCs) can secrete lots of protective factors that in turn promote function recovery. In the present study, we assessed the potential effects of MSCs conditioned medium (MSC-CM) against chronic ethanol-associated damage on PC12 cells and primary cortical neurons. We found that pretreatment with MSC-CM notably improved cell survival, prevented chronic ethanol-associated apoptosis and abolished the robust deterioration in oxidative status. In addition, we also discovered that chronic ethanol exposure induced an inactivation of phosphatidylinositol-3-kinase (PI3K)/Akt and a lasting activation of extracellular signal-regulated kinase 1/2 (ERK1/2) in both PC12 cells and primary cortical neurons which were able to be reversed by MSC-CM. The PI3K inhibitor (LY294002) was able to reduce the antioxidative and cytoprotective effects conferred by MSC-CM, in part, and the ERK1/2 inhibitor (PD98059) was able to elicit significant protection from chronic ethanol cytotoxicity but not rescue the deterioration in oxidative status induced by chronic ethanol. Taken together, these findings provide the first evidence that MSCs might have potent antioxidant action to shield the apoptotic impairment from chronic ethanol exposure in PC12 cells and neurons, which is involved in upregulation of PI3K/Akt and modulation of ERK1/2 activation, at least partially.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 167, Issue 4, 2 June 2010, Pages 1115-1124
Journal: Neuroscience - Volume 167, Issue 4, 2 June 2010, Pages 1115-1124
نویسندگان
L. Liu, J.-X. Cao, B. Sun, H.-L. Li, Y. Xia, Z. Wu, C.-L. Tang, J. Hu,