کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6280391 | 1615095 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
ER and Golgi stresses increase ER-Golgi SNARE Syntaxin5: Implications for organelle stress and βAPP processing
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کلمات کلیدی
βAPPtunicamycinSTSBFAThapsigarginSNARESmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAenzyme linked immunosorbent assay - آنزیم تست ایمونوسیورسانس مرتبط استER stress - استرس استstaurosporine - استوسوسورپینbrefeldin A - برفلدین AAlzheimer's disease - بیماری آلزایمرELISA - تست الیزاDIV - دیوday in vitro - روز in vitrosarco/endoplasmic reticulum Ca2+-ATPase - سارکو / سلولهای اندوپلاسمی Ca2 + -ATPaseendoplasmic reticulum - شبکه آندوپلاسمی SERCA - قلبNeuron - نورونHippocampus - هیپوکامپ β-amyloid precursor protein - پروتئین پیش ماده β-آمیلوئیدAβ peptide - پپتید Aβamyloid-β peptide - پپتید آمیلیید ب
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: ER and Golgi stresses increase ER-Golgi SNARE Syntaxin5: Implications for organelle stress and βAPP processing ER and Golgi stresses increase ER-Golgi SNARE Syntaxin5: Implications for organelle stress and βAPP processing](/preview/png/6280391.png)
چکیده انگلیسی
Unresolved endoplasmic reticulum (ER) stress causes neuronal death and has been implicated in neurodegenerative conditions such as Alzheimer's disease (AD). However, the mechanisms by which stress signals propagate from the ER through the Golgi apparatus and their effects on the transport and processing of AD-related proteins, such as β-amyloid precursor protein (βAPP), are unknown. We recently found that in the NG108-15 cell line, ER stress upregulates ER-Golgi-soluble N-ethylmaleimide-sensitive factor-attachment protein receptors (ER-Golgi SNAREs) Syx5 and Bet1. In the present study, we examined the effects of apoptosis and ER stress inducers on the expression of ER-Golgi SNARE proteins and cell viability in a primary culture of rat hippocampal neurons. An apoptosis inducer significantly downregulated the expression of ER-Golgi SNARE Syx5. ER-stress inducers upregulated the expression of Syx5 isoforms and Bet1 proteins via de novo synthesis of their mRNA transcripts. Knockdown of Syx5 during apoptosis or ER stress induction enhanced vulnerability of neurons. Additionally, we examined the effects of Golgi stress on Syx5 expression and βAPP processing. Golgi stress also induced upregulation of ER-Golgi SNARE Syx5, and concomitantly, suppressed amyloid-β peptide secretion. These findings suggest that Syx5 is a potential stress responsive factor that participates in βAPP processing and the survival pathways of neuronal cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 604, 14 September 2015, Pages 30-35
Journal: Neuroscience Letters - Volume 604, 14 September 2015, Pages 30-35
نویسندگان
Kei Suga, Ayako Saito, Tatsuya Mishima, Kimio Akagawa,