کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6280883 | 1615108 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Up-regulation of glutamine synthesis in microglia activated with endotoxin
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کلمات کلیدی
HRPIBA1GLNGLT-1GLASTDulbecco’s modified essential mediumPKCαM-CSFTSPGFAPDMEMLPSPBSnuclear magnetic resonance - رزونانس مغناطیسی هستهایadenosine 5′-triphosphate - آدنوزین 5'-تری فسفاتATP - آدنوزین تری فسفات یا ATPglutamic acid - اسید گلوتامیکNMR - تشدید مغناطیسی هستهای lipopolysaccharide - لیپوپلی ساکاریدmacrophage colony-stimulating factor - ماکروفاژ عامل کلونی تحریک کنندهPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMicroglia - میکروگلیاهاHorseradish peroxidase - پراکسیداز هوررادیشGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالprotein kinase C alpha - پروتئین کیناز C آلفاGlu - گلوglutamate - گلوتاماتglutamate transporter-1 - گلوتامات transporter-1glutamine - گلوتامینGlutamine synthetase - گلوتامین سنتتاز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We previously verified that newborn rat brain-derived microglia have the ability to uptake 14C-glutamate (Glu) through glutamate transporter-1. A given amount of Glu incorporated into microglia was suspected to be metabolized to glutamine (Gln). However, the ability of microglia to do this had not been demonstrated. Thus, in the present study we examined the possibility that primary rat microglia metabolize Glu into Gln. Immunocytochemical and immunoblotting studies indicated that the microglia express glutamine synthetase (GS) protein. As expected from these results, GS activity was actually detected in microglia, although the specific activity was lower than that of astrocytes. Considering this microglial property, it seemed possible that the taken Glu is metabolized to Gln in the cells. To investigate this possibility, we exposed microglia to [13C]Glu-containing medium and analyzed the change of Glu to Gln in a nuclear magnetic resonance examination. The results clarified that non-stimulated microglia hardly changed Glu to Gln, but when stimulated with lipopolysaccharide the microglia significantly metabolized [13C]Glu to [13C]Gln. Microglia were thus, strongly suggested to metabolize Glu to Gln via GS activity when activated in the inflammatory/pathological state of the nervous system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 591, 30 March 2015, Pages 99-104
Journal: Neuroscience Letters - Volume 591, 30 March 2015, Pages 99-104
نویسندگان
Kazuyuki Nakajima, Tomoyuki Kanamatsu, Yosuke Takezawa, Shinichi Kohsaka,