کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6304030 | 1618414 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The effects of short- and long-term freezing on Porphyra umbilicalis Kützing (Bangiales, Rhodophyta) blade viability
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کلمات کلیدی
AWCRBCsdNTPSGRHSDPSIIPSIPAMNH4CldsDNApulse amplitude modulatedPorphyra umbilicalisDNA - DNA یا اسید دزوکسی ریبونوکلئیکFv/Fm - Fv / FmrbcL - rbclROS - ROSp-value - ارزش pdeoxyribonucleic acid - اسید deoxyribonucleicdouble-stranded deoxyribonucleic acid - اسید دز اکسید ریبونوکلئیک دو رشته ایFreezing - انجمادEcophysiology - اکوفیزیولوژیanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceDeoxynucleotide Triphosphates - تری فسفاتهای دیوکسینوکوتیدhonestly significant difference - تفاوت صادقانه قابل توجه استhigh sensitivity - حساسیت بالاPreservation - حفاظتCarbon dioxide - دیاکسید کربنViability - زنده ماندنZonation - زونphotosystem I - عکس سیستم منPhotosystem II - فتوسیستم 2Magnesium - منیزیمspecific growth rate - نرخ رشد خاصNew Hampshire - نیوهمپشایرVSE - همهpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازnatural log - ورود طبیعیCO2 - کربن دیاکسیدAmmonium chloride - کلرید آمونیومReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Seaweeds inhabiting the upper intertidal zone are subjected to temperature, light, and water stresses and vertical distribution has been linked to environmental tolerance. Previous studies have also attributed successful recovery from freezing stress in intertidal seaweeds to desiccation tolerance. Porphyra umbilicalis Kützing is an aseasonal red alga inhabiting the mid to upper intertidal zone in temperate and subarctic regions of the North Atlantic. It is a member of the economically important group of foliose Bangiales, and has been documented to only reproduce asexually via neutral spores in the Northwest Atlantic. The goal of this study was to assess the effects of freezing on the viability of small blades of P. umbilicalis. Cultured blades of P. umbilicalis (4.8 ± 0.22 mg) were air dried to 5% or 30% absolute water content (AWC) and frozen for 1, 3, 6, or 12 months at â 80 °C or â 20 °C. Following freezing, blades were rehydrated and the growth rate of each blade was measured weekly for 4 weeks. Photosynthetic efficiency of photosystem II (Fv/Fm) was assessed for each blade 3 h and 4 weeks post-rehydration. Overall, there was 100% blade survival and all blades continued to grow after rehydration. Although the conditions under which the blades were frozen did have statistically significant effects on post-rehydration growth rate and Fv/Fm, in general the differences were quite small. Post-rehydration growth rates ranged from 7.06 to 8.03 ± 0.16% dayâ 1. AWC had an effect on post-rehydration growth rates for blades frozen at â 80 °C, but not blades frozen at â 20 °C. The length of freezing had a somewhat greater effect on blades with 5% AWC than blades with 30% AWC. Growth rates peaked two weeks post-rehydration followed by a small decline in weeks 3 and 4. Fv/Fm values following freezing were generally similar to those recorded in previous studies on non-frozen blades; however, blades frozen for 6 months performed better than blades frozen for 12 months. Overall, these results indicate that short- and long-term freezing have little physiological effect on blades of P. umbilicalis. Therefore, freezing may be a viable method for preservation of P. umbilicalis for aquaculture.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Experimental Marine Biology and Ecology - Volume 461, December 2014, Pages 499-503
Journal: Journal of Experimental Marine Biology and Ecology - Volume 461, December 2014, Pages 499-503
نویسندگان
Lindsay A. Green, Christopher D. Neefus,