کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6390122 | 1628395 | 2016 | 9 صفحه PDF | دانلود رایگان |
- A full-validated LC-MS method for detecting eight PAs in honey was developed.
- A simple, environmentally friendly extraction was performed.
- Despite their potential health consequences, no regulatory levels were settled.
- At least three PAs were detected in 99.07% of the 92 samples.
- Several analysed samples exceeded the suggested tolerable daily doses for PAs.
A fast and simple method was developed to determine pyrrolizidine alkaloids (PAs) in honey using liquid chromatography tandem mass spectrometry (LC- MS/MS) with electrospray ionization (ESI). An efficient extraction procedure was carried out by simply diluting with water, without the need of any additional clean-up steps. A full validation of the method was performed according to Commission Decision 2002/657/EC. The method was linear in the 050 μg kgâ1 range and presented satisfactory intra-day and inter-day precision with relative standard deviations of 1.45-10.2% and 1.60-1-0.2%, respectively. The measurement uncertainty, limit of detection (LOD) (0.1-1.0 μg kgâ1) and limit of quantification (LOQ) (0.2-1.5 μg kgâ1) were also calculated. The proposed method was applied to analyse eight PAs, namely, senecionine, senecionine-N-oxide, echimidine, intermedine, lycopsamine, retrorsine, monocrotaline and retrorsine-N-oxide, in 92 commercial honey samples from Brazil. At least three PAs were detected in 99.1% of the samples. PAs were not detectable ( 149
Journal: Food Control - Volume 67, September 2016, Pages 183-191