کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6390239 1628402 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inactivation of Escherichia coli O157:H7 in vitro and on the surface of spinach leaves by biobased antimicrobial surfactants
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Inactivation of Escherichia coli O157:H7 in vitro and on the surface of spinach leaves by biobased antimicrobial surfactants
چکیده انگلیسی


- Biosurfactants were tested against Escherichia coli O157:H7 in vitro and on spinach leaves.
- Sophorolipid and thiamine dilauryl sulfate inactivated E. coli O157:H7 in vitro.
- Thiamine dilauryl sulfate reduced E. coli population on spinach by 3.1 log CFU/leaf.
- Bio-based antimicrobial surfactants have a potential for use to enhance produce safety.

This study was conducted to evaluate the effect of biosurfactants on the populations of Escherichia coli O157:H7 in suspension and on spinach leaves. Eight surfactants including four soybean oil-based biosurfactants, sodium dodecyl sulfate (SDS), polyoxyethylene sorbitan monooleate (Tween 80), sophorolipid (SO) and thiamine dilauryl sulfate (TDS) at concentrations of 0.1%, 0.5% and 1.0% were tested in bacterial suspension, and the most effective biosurfactants were applied on spinach leaves. Results showed that the soybean oil-based biosurfactants, SDS or Tween 80 did not significantly affect E. coli O157:H7 populations. SO and TDS at concentrations of 1.0% were effective in reducing E. coli O157:H7 populations in bacterial suspension. E. coli O157:H7 with an initial population of 7.1 log CFU/mL was not detectable (detection limit: 1 log CFU/mL) after 1 min in 1.0% TDS or after 2 h in 1.0% SO. On spinach leaves, SO at 1% did not significantly affect E. coli when compared to a water wash during 7 days post-treatment storage at 4 °C. However, TDS (1.0%) wash was as effective as 200 ppm chlorine in reducing population of spot inoculated E. coli O157:H7, achieving 3.1 and 2.7 log CFU/per leaf at day 0, and 1.4 and 1.9 log CFU/leaf at day 7 when compared with a water wash. No apparent change in spinach visual quality was observed. None of treatments caused changes in visual quality of spinach. Electron micrographs suggested ultrastructural damage of bacterial cells such as separation of the outer membrane from the cytoplasmic membrane. Overall, our results showed that SO and TDS may be potential sanitizers in inactivating human pathogens such as E. coli O157:H7 in wash water and on fresh produce.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 60, February 2016, Pages 158-165
نویسندگان
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