کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6390600 1628403 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of poultry species using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) methods
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Identification of poultry species using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) methods
چکیده انگلیسی


- PCR-SSCP and CE-SSCP methods were developed for poultry species identification in raw meat and processed meat products as well.
- Detection limit of the assays was 0.5%.
- Fraud was revealed, 6 of 36 meat products contained undeclared species.
- Assays are sensitive, reproducible and reliable.

In the last decades, animal species identification became more important to prevent food adulteration. Here, we demonstrate the identification of seven poultry species, chicken, guinea fowl, pheasant, turkey, goose, duck and muscovy duck, through the use of the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) methods. DNA were isolated from poultry meat and meat products and were amplified with universal primers, designed for the mitochondrial 12S rRNA. Species-specific patterns and the reliable detection limit were identified as 0.5% for PCR and CE applications. Analyses of commercially available poultry products revealed fraud, as 6 of 36 contained undeclared species. The above-mentioned techniques are sensitive, reproducible and reliable for poultry species identification from foodstuffs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 59, January 2016, Pages 430-438
نویسندگان
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